Fig. 6.

ERK1/2 activity is augmented in vessels from TNF-α-infused IL-10^−/− mice. A and B: representative immunoblots for total ERK1/2, phosphorylated ERK1/2, and β-actin expression in murine aorta (top) and corresponding bar graphs (bottom) demonstrating regulatory role of IL-10 on total and phosphorylated ERK1/2. C: ratio of phosphorylated to total ERK1/2 vascular proteins. Densitometric analysis was performed in samples from vehicle- and TNF-α-infused WT and vehicle- and TNF-α-infused IL-10^−/− mice. Values, expressed in arbitrary units, are means ± SE (n = 5 experiments) and were normalized to β-actin protein expression. *P < 0.05 vs. respective control (i.e., WT). †P < 0.05 vs. respective vehicle-infused group.