Fig. 2.

Reaction of anaerobic deoxy-sHbF and -sHbA with 1 mM nitrite in the absence of dithionite. A and B: disappearance of sheep adult and fetal deoxy-Hb and the formation of iron nitrosyl hemoglobin and total methemoglobin (Tot Met)-Hb over time in the reaction of 50 μM deoxy-Hb with 1 mM nitrite at pH 7.4 and 25°C. C: instantaneous bimolecular rate constants over time for the reaction in A and B calculated as the instantaneous reaction rate divided by the concentrations of deoxy-Hb and nitrite at that instant.