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Effect of nitrite concentration on the rate of reaction of sHbA and sHbF
| [NO2−], mM |
t1/2, s |
|
|---|---|---|
| sHbA | sHbF | |
| 1.0 | 2,621±330 | 1,168±153 |
| 2.5 | 1,120±104 | 572±38 |
| 5 | 673±56 | 208±13 |
| 10 | 202±11 | 97±5 |
Data are means ± SD of 3 separate mixing experiments. The observed time (t1/2) corresponding to the time required to convert one-half of the reactant, deoxy-Hb, to the product, iron nitrosyl hemoglobin, is inversely proportional to the concentration of nitrite.
