Fig. 6.

Decreased OGT expression in NRVMs reduced O-GlcNAc levels and enhanced LPS-induced increase in IκB-α phosphorylation. A: immunoblots of O-GlcNAc, OGT, P- and T-IκB-α. B: mean densitometric analysis of O-GlcNAc and OGT normalized to GAPDH. C: mean densitometric analysis of P/T-IκB-α. Cells were transfected with either OGT siRNA (si-OGT) or scrambled siRNA (si-Neg) 48 h before treatment with LPS (2 μg/ml) for 6 h. Data are normalized to Si-Neg group and presented as means ± SE of n = 5 experiments per group. *P < 0.05 vs. Con; #P < 0.05 vs. LPS treated.