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Glucosamine treatment of RAW 264.7 cells increased cardiac O-GlcNAc levels and attenuated LPS-induced P-IκB-α and inducible nitric oxide synthase (iNOS) protein expression. Cells were pretreated with 5 mM glucosamine for 30 min and before treatment with LPS (0.1 μg/ml, 6 h). C, control; L, LPS treated.
