Fig. 3.

Effects of PGE2, IL-8, and RhoA on wt and mutant PAI-2 promoter constructs. A: flow cytometric analysis shows that gastrin increased COX-2 and IL-8 in AGS cells expressing the CCK-2 receptor. B: Western blot shows that the abundance of wt PAI-2 protein is increased in AGS cells treated with PGE₂ and IL-8. C: PGE₂ (28 μM, 6 h) and IL-8 (125 ng/ml, 6 h) stimulated expression of wt PAI-2-93-luc. D: responses to PGE-2 are significantly reduced in m10 and m11 but not m5, whereas the response to IL-8 is reduced in m5 but not in m10 and m11. E: cotransfection of AGS cells with L63RhoA (0.5 μg/well) stimulates PAI-2-93-luc to a lesser extent than PAI-2-196 (*P < 0.05, t-test, n = 3). F: responses to L63RhoA of PAI-2-93-luc were significantly reduced by mutations of the MAZ site (m10-11) but not of the GACAGA site (m5). *P < 0.05, ANOVA; n = 3.