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. 2008 Dec 12;296(2):G414–G423. doi: 10.1152/ajpgi.90340.2008

Fig. 4.

Fig. 4.

Analysis of the MAZ binding site. A: wt and mutant MAZ double-stranded (ds) oligonucleotides (sequences inset, 100× excess) were used to compete with radiolabeled wt probe binding to nuclear extracts from G17-stimulated AGS-GR cells. MAZ site-specific binding complexes are indicated by arrows. Left, whole gel; right, enhancement of MAZ site-specific complexes. B: effects of transcription factor antibodies on wt probe binding to nuclear extracts from G17-stimulated AGS-GR cells. Lane 1, no antibody, lane 2, no extract, lanes 3-12, antibodies as indicated. C: PCR analysis of chromatin immunoprecipitation (ChIP) assay. Lane 1, positive (+ve) control primers (143-bp amplicon) with MAZ immunoprecipitated DNA as template. Lane 2, positive control primers with IgG immunoprecipitated DNA as template. Lane 3, positive control primers with unprecipitated input DNA as template. Lane 4, 100-bp marker. Lanes 5-7, templates as for lanes 1-3 but with negative (−ve) control primers (186-bp amplicon).