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. 2008 Nov 25;296(2):G135–G146. doi: 10.1152/ajpgi.90462.2008

Fig. 12.

Fig. 12.

Adoptive transfer of CD4+ T cells obtained from interleukin-10-deficient (IL-10−/−) mice induces chronic colitis in RAG−/− recipients at 8 wk posttransfer. IL-10−/− splenocytes were enriched for CD4+ T cells (>85%) using a commercially available negative selection kit specifically for CD4+ T cells (Dynal). Wild-type (WT) CD4+CD45RBhigh T cells were purified by flow cytometry as described in the methods. RAG-1−/− recipients were injected (ip) either with IL-10−/− CD4+ T cells (1 × 106 cells) or with CD4+CD45RBhigh T cells (0.5 × 106 cells). Note that IL-10−/− CD4+ injected mice possessed a similar incidence and severity of chronic colitis as did RAG−/− mice injected with WT naive T cells purified by flow cytometry. n = 8 for each group.