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A and B: RT-PCR shows significantly higher levels of COX-1 and COX-2 in IAS than in rectal smooth muscle (RSM) from rat. Data were normalized to β-actin levels. C and D: Western blot analyses show higher expression of COX-1 in IAS than RSM. Data were normalized to α-actin levels. Values are means ± SE. *P < 0.05.
