Figure 4.

Evolutionary constraints on protein sequence and structure features. Error bars represent 95th percentile confidence intervals from bootstrap resampling. (a) For proteins shorter than 500 residues, short proteins have high A/S ratios. (b) Buried residues are under stronger selection. The 95th percentile confidence intervals of the A/S ratio are [0.23, 0.25] for buried residues, and [0.30, 0.32] for exposed residues. (c) Loop residues have relaxed evolutionary constraints. The 95th percentile confidence intervals of the A/S ratio are [0.25, 0.26] for residues in alpha-helices, [0.24, 0.27] for residues in beta-strands, and [0.30, 0.32] for residues in loops. (d) Proteins with disordered regions are more conserved, while disordered residues are under lower selective pressure. (e) Residues in low complexity regions evolve faster.