Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2008 Nov 8;79(3-5):123–129. doi: 10.1016/j.plefa.2008.09.021

Figure 2.

Figure 2

Representative fatty acid calibration curve of saturated and unsaturated fatty acids. Fatty acid standard curves were prepared for each fatty acid by injection of 1 μl of calibration standard mixture containing 0.15 ng to 500 ng of each fatty acid (0.003 ng/μl to 10 ng/μl). For regression analysis, the ratio of unlabeled fatty acid/deuterated fatty acid (H18:0/D18:0, H20:4/D20:4) was calculated as described under “Materials and Methods” and plotted against the absolute amount of unlabeled fatty acid (H18:0, H20:4) in the standard mixture. Inset shows part of the calibration curve at low analyte concentration range. Linear regression parameters were computed and used for quantitative analyses of endogenous fatty acids.