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Representative immunofluorescent labeling of the medial (me) and intermediate (im) subnuclei of the nucleus tractus solitarii (NTS) on the left side where sham treatment was given (A and C) and on the right side where substance P conjugated with saporin (SSP-SAP) had been injected (B and D). Note marked reduction in immunostaining for neurokinin-1 (NK1) receptors in the NTS on the treated side. Scale bar = 20 μm.
