Table 1.

[leucyl-³H]N/OFQ binding in CNS tissues isolated from male Sprague-Dawley rats maintained on either a chronic (3-week) a normal (0.4%) or high (8%)-NaCl chow

	PKD		Bmax, fmol NOP Receptor/mg Protein
	Normal Diet, 0.4% NaCl	High-Salt Diet, 8% NaCl	Normal Diet, 0.4% NaCl	High-Salt Diet, 8% NaCl
Brain cortex	9.09±0.03	9.43±0.07	416±45	218.1±22*
Hypothalamus	9.43±0.04	9.27±0.14	712.1±35	883.2±49*
Medulla	9.14±0.12	9.07±0.04	1473±131	326.5±31†

Data are means ± SE from 6 individual experiments. N/OFQ, nociceptin/orphanin FQ; NOP, N/OFQ peptide; CNS, central nervous system; B_max, maximum binding capacity;

^*P < 0.05,

^†P < 0.01, statistically different from normal chow value.