Fig. 8.
IL-17A alkalinizes surface pH in HBE cells. SNARF-1 dextran (1 mg/ml in perfluorocarbon) was added to the mucosal surface of mature HBE cells, and the F640-to-F580 ratio was measured every min. A: calibration of SNARF-1 dextran in aqueous solutions of pH 6.5, 7, and 7.5. B: surface pH measurements from 8 vehicle (open circles) and IL-17A-treated (filled circles) filters representing 3 donors. Note that resting surface pH is more alkaline in IL-17A-treated cells than controls. Horizontal bar represents group mean. *P < 0.05 by Mann-Whitney.