Fig. 9.

Intracellular pH is not different in IL-17A-treated cells vs. controls. Mature HBE cells were loaded with SNARF-5-AM before mounting on the microscope stage. Cells were overlayed with 100 μl of HCO₃⁻-buffered solution and kept in the presence of 95% O₂-5% CO₂. Cells were perfused on the serosal side with CO₂-gassed HCO₃⁻-buffered solution. F₆₄₀/F₅₈₀ was measured every min until stability after which the serosal solution was changed to one containing 2 μM forskolin. A: calibration of intracellular pH using SNARF-5-AM after equilibration in solutions of pH 6.5, 7, and 7.5. B: mean intracellular pH ± SE in controls (open circles) vs. IL-17A-treated cells (filled circles) (n = 8 filters from 3 donors). There is no difference between IL-17A-treated cells and controls in either baseline pH or change in pH with forskolin.