Table 1.
Quantities of uPA and other proteins in day-7 and day-13 QPD and control megakaryocyte cultures, evaluated by ELISA
| Protein, unit/sample | n | Mean ± SEM (range)/106 cells |
||
|---|---|---|---|---|
| Secreted |
Cell associated |
|||
| Day 7 | Day 13 | Day 13 | ||
| uPA, pg | ||||
| Q | 4 | 460 ± 50 (320-550)* | 16 000 ± 4,000 (7000-26 000)*† | 4700 ± 2000 (670-10 000)* |
| C | 4 | 160 ± 80 (50-370) | 150 ± 90 (40-440) | 20 ± 7 (3-40) |
| PAI-1, ng | ||||
| Q | 4 | < 0.05 | 63 ± 19 (31-109) | 25 ± 7 (7-43) |
| C | 4 | 1 ± 1 (<0.05-3) | 68 ± 21 (16-105) | 25 ± 8 (11-48) |
| uPA-PAI-1 complexes, pg | ||||
| Q | 4 | < 100 | 500 ± 300 (< 100-1100) | 600 ± 300 (< 100-1300) |
| C | 4 | < 100 | < 100 | < 100 |
| PF-4, IU | ||||
| Q | 3 | < 0.3 | 148 ± 60 (27-210) | 317 ± 146 (31-513) |
| C | 3 | < 0.3 | 131 ± 46 (39-185) | 245 ± 120 (46-460) |
| TSP-1, % NPP | ||||
| Q | 3 | < 0.03 | 2.3 ± 0.6 (1.1-3.2) | 2.2 ± 0.8 (0.6-3.1) |
| C | 3 | < 0.03 | 3.4 ± 1.5 (0.8-6.0) | 5.0 ± 2.2 (1.7-9.1) |
| MMRN1, ng | ||||
| Q | 6 | < 25 | 25 ± 20 (< 25-147) | 154 ± 36 (33-260) |
| C | 6 | < 25 | 105 ± 39 (< 25-232) | 255 ± 99 (75-761) |
| VWF, ng | ||||
| Q | 3 | < 8 | 140 ± 36 (75-202) | NE |
| C | 3 | < 8 | 85 ± 36 (21-145) | NE |
Q indicates QPD; C, control; NPP, normal pooled platelets; and NE, not evaluated.
*
P < .05 for comparisons of Q versus C.
†
P < .05 for comparisons of day-13 versus day-7 samples.