Fig. 5.

c-kit^BAC-EGFP neonatal heart cells are cardiovascular precursors. (A) Modification of commitment outcomes by addition of specific growth factors. Number of experiments indicated in bars. (B) Clonal expansion showing a single EGFP⁺ cell at day 3 (Left) and the clone after 20 days (Right). (C) Immunostaining of clones from a single EGFP⁺ cell after 21 days. Individual cells showing commitment to all 3 heart lineages. (D) Coimmunostaining of clonal populations demonstrates commitment to cardiac (Left) or smooth muscle (Right) and endothelium in the same clone. DAPI staining (blue) in C. (Scale bars: B–D, 50 μm.)