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. 2009 Feb 2;106(6):1936–1941. doi: 10.1073/pnas.0812904106

Fig. 4.

Fig. 4.

Characterization of the anti-CDX1 mAb. (A) Peptide-blocking immunoblot of the anti-CDX1 mAb. CDX1 mAb was incubated either with N-term or carboxyl peptides or PBSA before immunoblotting with lysates from either T: HCT116-CDX1 or C: HCT116-Vec. (B) Immunoblot of CDX1-expressing and -nonexpressing CRC cell lines. (C) Immunoblot of the 2 pairs of stably transfected CRC cell lines, HTC116-Vec and HCT116-CDX1, and LS174T-Vec and LS174T-siRNA using anti-CDX1 and anti-KRT20 monoclonal antibodies. Anti β-tubulin was used as a loading control in (A), (B), and (C). (D) Immunohistochemistry using the anti-CDX1 and anti-KRT20 mAbs on the CDX1-expressing cell line LS174T and the non-expressing cell line HCT116.