TABLE 1.
FEATURES OF MALIGNANT PLEURAL EFFUSIONS GENERATED IN C57B/6 MICE 14 d AFTER INTRAPLEURAL INJECTION OF 15 × 105 LLC CELLS (n = 9) COMPARED WITH UNTREATED MICE (n = 5).
| Features | Untreated Mouse | Mouse MPE |
|---|---|---|
| Pleural fluid volume, μl | 16.2 ± 1.4 | 667 ± 58 |
| Pleural surface tumor number | 0 | 8.6 ± 1.0 |
| Red blood cells, ×103/μl | ||
| Pleural fluid | 0 | 4.7 ± 0.55 |
| Blood | 8.2 ± 0.7 | 8.4 ± 0.15 |
| Nucleated cells, ×103/μl | ||
| Pleural fluid | 0.5 ± 0.2 | 32.7 ± 4.5 |
| Blood | 6.5 ± 1.3 | 8.0 ± 0.6 |
| Pleural fluid tumor cells, ×103/μl | 0 | 14.7 ± 2.9 |
| Pleural fluid cells, % of nontumor cells | ||
| Mononuclear | 72 ± 12 | 57 ± 5 |
| Lymphocytes | 22 ± 5 | 23 ± 6 |
| Neutrophils | 5 ± 3 | 13 ± 4 |
| Eosinophils | 1 ± 1 | 12 ± 2 |
| Basophils | Occasional | Occasional |
| Glucose, mg/dl | ||
| Pleural fluid | 167 ± 15 | 208 ± 55 |
| Serum | 87 ± 5 | 214 ± 73 |
| Protein, g/dl | ||
| Pleural fluid | 1.07 ± 0.1 | 2.18 ± 0.03 |
| Serum | 1.76 ± 0.31 | 1.67 ± 0.15 |
| Ratio | 0.6 ± 0.06 | 1.37 ± 0.14 |
| LDH, U/l | ||
| Pleural fluid | 1,204 ± 105 | 6,850 ± 1,170 |
| Serum | 995 ± 204 | 1,213 ± 147 |
| Ratio | 1.21 ± 0.12 | 5.57 ± 0.62 |
| VEGF, pg/ml | ||
| Pleural fluid | 88 ± 52 | 2,303 ± 284 |
| Serum | 64 ± 47 | 47 ± 29 |
| Vascular hyperpermeability, pleural fluid/serum Evan's blue | No (< 1%) | Yes (26 ± 9%) |
Definition of abbreviations: LDH, lactate dehydrogenase; LLC, Lewis lung cancer; MPE, malignant pleural effusion; VEGF, vascular endothelial growth factor.
Values represent mean ± SEM.