Figure 2.

Ozone produces toxicity but no chemokine secretion. Cells were cultured and exposed to air (control [ctrl]) or to ozone 100 ppb for 60 min and cultured for an additional 24 h. (A) DNA/well, which was selected as a marker of toxicity as a measure of cell detachment. (B and C) Secretion of the chemokines MCP-1 and MIP-2 into the media. (D) 8-Isoprostane levels in the medium as a marker of lipid peroxidation. The values are normalized to the air control, and values represent mean ± SEM for five different experiments. *P < 0.05 compared with control.