Figure 7.

Pretreatment with LPS does not alter the ozone effect on the type I–like phenotype. Type I–like cells were primed with 10 ng /ml of LPS for 18 h before filtered air or ozone exposure. Cells were cultured in fresh media for 24 h after exposure and before collection of media and cells for different assays. (A) Cell by DNA/well, which is a measure of cell detachment or toxicity. (B and C) MCP-1 and MIP-2 secretion. (D and E) Nitrite and 8-isoprostane release into the medium. Each value represents the mean ± SEM for three experiments. *P < 0.05 for the untreated ozone exposure compared with air. The pound sign (#) signifies the effect of LPS compared with the appropriate air or ozone controls (P < 0.05).