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LPS stimulates type II cells to secrete MCP-1 and MIP-2. Type II cells were stimulated with increasing concentrations of LPS (10 ng/ml– 1 μg/ml) for 18 h. Medium was changed, and cells were cultured for 24 h before harvest. (A and B) Amount of MCP-1 and MIP-2 secreted in response to LPS. (C) Increase in nitrite in the medium. Each value represents the mean ± SEM for n = 3. #P < 0.05 compared with control.
