Figure 9.

Pretreatment with LPS does not alter the ozone effect on the type II cell phenotype. Type II cells were primed with 10 ng /ml of LPS for 18 h before filtered air or ozone exposure. Cells were grown in fresh media for 24 h after exposure and before collection of media and cells for different assays. (A) Cell DNA/well, which is a measure of cell attachment or toxicity. (B and C) MCP-1 and MIP-2 secretion. (D and E) Nitrite and 8-isoprostane release into the medium. Each value represents mean ± SEM for n = 3. *P < 0.05 for the untreated ozone exposure compared with air. The pound sign (#) signifies the effect of LPS compared with the appropriate air or ozone control (P < 0.05).