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. 2005 Sep 29;34(1):108–118. doi: 10.1165/rcmb.2005-0164OC

Figure 2.

Figure 2.

Figure 2.

Figure 2.

Figure 2.

Allograft lymph node (LN) and spleen produce alloantigen-specific IFN-γ in response to allogeneic BALB/c spleen at Days 14 and 28 after transplant. Allograft (BALB/c into B6) or isograft (B6 into B6) LN (A, B) and spleen (C, D) were cultured (see MATERIALS AND METHODS). At 72 h, culture supernatants were assessed using ELISA for IFN-γ (limit of assay, 37 pg/ml). Values represent the mean of the samples performed in triplicate, and the error bars represent the SEM. Statistically significant differences between allograft cells stimulated with BALB/c sp. compared with medium or syngeneic spleen or isograft cell cultures are indicated by (*P ⩽ 0.01 in [A], P ⩽ 0.02 in [B], P ⩽ 0.05 in [C], P ⩽ 0.001 in [D]), and with Con A–stimulated cultures by (**P ⩽ 0.0001 in [A], P ⩽ 0.0001 in [B], P ⩽ 0.0001 in [C], P ⩽ 0.001 in [D]), whereas (P ⩽ 0.03 in [A], P ⩽ 0.0001 in [C]) and (P ⩽ 0.03 in [A], P ⩽ 0.0001 in [C]) indicate significant differences between Day 14 compared with Day 28 alloantigen and Con A responses, respectively. Results are representative of eight independent experiments (n = 3–5 mice per group).