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. 2005 Sep 29;34(1):108–118. doi: 10.1165/rcmb.2005-0164OC

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Figure 3. — Both CD4⁺ and CD8⁺ T cells contribute to alloantigen-specific IFN-γ production in allograft spleen and LN. Pooled Day 14 cells from allograft (BALB/c into B6 wild-type) spleen (A) and allograft (BALB/c into CD45.1 congenic B6) or isograft (B6 into CD45.1-congeneic B6) draining LN (B) cells were cultured in 48-well plates (see MATERIALS AND METHODS), and CD45.1⁺ gated CD4⁺ (FITC-conjugated) and CD8⁺ (PerCP Cy5.5–conjugated) populations were analyzed after staining with APC-conjugated anti–IFN-γ or isotype control Ab after ICS. Numbers in upper right of panels are the percentage of positive staining cells for gated populations. Results are representative of three separate experiments (n = 3–5 mice per group).

Figure 3. — Both CD4⁺ and CD8⁺ T cells contribute to alloantigen-specific IFN-γ production in allograft spleen and LN. Pooled Day 14 cells from allograft (BALB/c into B6 wild-type) spleen (A) and allograft (BALB/c into CD45.1 congenic B6) or isograft (B6 into CD45.1-congeneic B6) draining LN (B) cells were cultured in 48-well plates (see MATERIALS AND METHODS), and CD45.1⁺ gated CD4⁺ (FITC-conjugated) and CD8⁺ (PerCP Cy5.5–conjugated) populations were analyzed after staining with APC-conjugated anti–IFN-γ or isotype control Ab after ICS. Numbers in upper right of panels are the percentage of positive staining cells for gated populations. Results are representative of three separate experiments (n = 3–5 mice per group).