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. 2005 Sep 29;34(1):108–118. doi: 10.1165/rcmb.2005-0164OC

Figure 5.

Figure 5.

Figure 5.

Figure 5.

Lung mononuclear cells (LMNC) produce alloantigen-specific IFN-γ. On Day 14 after transplant, allograft (BALB/c into B6) and isograft (B6 into B6) LMNC were isolated and cultured (see MATERIALS AND METHODS). At 72 h, culture supernatants were assessed for IFN-γ using ELISA. Statistically significant differences between alloantigen-induced IFN-γ in allograft cultures compared with medium alone or isograft cultures are indicated by (*P ⩽ 0.001 in [A]). Differences in Con A–induced IFN-γ between allograft and isograft cultures are indicated by (**P ⩽ 0.04 in [A]). (B) Day 14 allograft (BALB/c into B6) LMNC cultures were stimulated similar to above, and gated CD4+ (PE-conjugated) and CD8+ (FITC-conjugated) populations were stained with APC-conjugated anti–IFN-γ or isotype control Ab. Allograft and Isograft CD8+ LMNC were also assessed for CD44 expression (C). Numbers in upper right of panels are the percentage of positive staining cells for gated populations. Results are representative of five separate experiments (n = 3–5 mice per group).