TABLE 2.
EXPRESSION DIFFERENCES IN GENES INVOLVED IN PRE- AND POST-TRANSLATIONAL PROCESSES IN THE LUNGS OF GENE-TARGETED MICE LACKING METALLOTHIONEIN 1 AND 2 [Mt1/2(−/−)] AND 129S1/SvImJ STRAIN-MATCHED WILD-TYPE MICE [Mt1/2(+/+)] EXPOSED TO NICKEL FOR 3 h
| Accession ID | Gene Name | Symbol | GO Biological Process | Mt1/2(+/+) Fold Change | Mt1/2(+/+) Fold Change | Mt1/2(−/−)/ Mt1/2(+/+) Ratio |
|---|---|---|---|---|---|---|
| NM_011664 | Ubiquitin B | Ubb | Protein modification | −2.3 ± 0.6 | +1.1 ± 0.3 | — |
| NM_018875 | Sorting nexin 12 | Snx12 | Protein transport | −2.2 ± 0.6 | +2.5 ± 1.4 | — |
| NM_011883 | Ring finger protein 13 | Rnf13 | Protein ubiquitination | −3.9 ± 1.2 | −1.5 ± 0.4 | 2.5 |
| NM_024267 | Importin 4 | Ipo4 | Protein transport | −4.1 ± 1.6 | −1.7 ± 0.8 | 2.4 |
| NM_026768 | Mitochondrial ribosomal protein S18A | Mrps18a | Protein biosynthesis | −2.6 ± 0.5 | −1.1 ± 0.2 | 2.3 |
| NM_018753 | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide | Ywhab | Protein targeting | −2.7 ± 0.9 | −1.1 ± 0.4 | 2.3 |
| NM_007597 | Calnexin | Canx | Protein folding | −2.7 ± 0.9 | −1.2 ± 0.3 | 2.2 |
| NM_013876 | Ring finger protein 11 | Rnf11 | Protein ubiquitination; ubiquitin-dependent protein catabolism | −2.4 ± 0.7 | −1.1 ± 0.3 | 2.2 |
| NM_145486 | Membrane-associated ring finger (C3HC4) 2 | March2 | Protein ubiquitination | −2.4 ± 0.5 | −1.1 ± 0.2 | 2.1 |
| NM_020600 | Ribosomal protein S14 | Rps14 | Protein biosynthesis; ribosome biogenesis | −2.2 ± 0.5 | −1.1 ± 0.2 | 2.0 |
| NM_019865 | Ribosomal protein L36a | Rpl36a | Protein biosynthesis; ribosome biogenesis | −3.8 ± 1.7 | −1.9 ± 0.9 | 2.0 |
| NM_030743 | Zinc finger protein 313 | Zfp313 | Protein ubiquitination | −2.5 ± 0.8 | −1.3 ± 0.4 | 1.9 |
| NM_013923 | Ring finger protein (C3HC4 type) 19 | Rnf19 | Protein ubiquitination | −2.3 ± 0.6 | −1.2 ± 0.3 | 1.9 |
| NM_013787 | S-phase kinase-associated protein 2 (p45) | Skp2 | Ubiquitin cycle | −6.3 ± 2.2 | −3.3 ± 1.7 | 1.9 |
| NM_023651 | Peroxisomal biogenesis factor 13 | Pex13 | Intracellular protein transport | −2.1 ± 0.5 | −1.2 ± 0.3 | 1.8 |
| NM_022997 | Vacuolar protein sorting 35 | Vps35 | Protein transport | −2.2 ± 0.6 | −1.3 ± 0.4 | 1.8 |
| NM_025586 | Ribosomal protein L15 | Rpl15 | Protein biosynthesis | −2.2 ± 0.5 | −1.3 ± 0.3 | 1.7 |
| AF100956 | Zinc finger protein 297 | Zfp297 | Protein folding | −2.0 ± 0.6 | −1.2 ± 0.3 | 1.7 |
| NM_024478 | GrpE-like 1, mitochondrial | Grpel1 | Protein folding | −2.3 ± 0.5 | −1.4 ± 0.3 | 1.7 |
| NM_009078 | Ribosomal protein L19 | Rpl19 | Protein biosynthesis | −2.5 ± 0.6 | −1.5 ± 0.4 | 1.7 |
| NM_138946 | Ribosomal protein S18 | Rps18 | Protein biosynthesis | −2.3 ± 0.4 | −1.4 ± 0.3 | 1.6 |
| NM_011187 | Proteasome (prosome, macropain) subunit, beta type 7 | Psmb7 | Ubiquitin-dependent protein catabolism | −2.1 ± 0.4 | −1.3 ± 0.3 | 1.6 |
| NM_007475 | Acidic ribosomal phosphoprotein P0 | Arbp | Ribosome biogenesis and assembly | −2.7 ± 0.7 | −1.7 ± 0.4 | 1.6 |
| NM_026693 | Gamma-aminobutyric acid (GABA-A) receptor-associated protein-like 2 | Gabarapl2 | Intracellular protein transport | −3.6 ± 1.1 | −2.4 ± 0.8 | 1.5 |
| NM_019727 | Sorting nexin 1 | Snx1 | Intracellular protein transport | −2.9 ± 0.9 | −1.8 ± 0.7 | 1.5 |
| NM_021433 | Syntaxin 6 | Stx6 | Intracellular protein transport | −2.1 ± 0.5 | −1.4 ± 0.5 | 1.5 |
| NM_025481 | SMAD specific E3 ubiquitin protein ligase 2 | Smurf2 | Ubiquitin cycle | −2.0 ± 0.5 | −1.3 ± 0.3 | 1.5 |
| NM_013911 | F-box and leucine-rich repeat protein 12 | Fbxl12 | Ubiquitin cycle | −2.4 ± 0.6 | −1.7 ± 0.4 | 1.5 |
| NM_009082 | Ribosomal protein L29 | Rpl29 | Protein biosynthesis; ribosome biogenesis | −2.0 ± 0.6 | −1.3 ± 0.3 | 1.5 |
| NM_012052 | Ribosomal protein S3 | Rps3 | Protein biosynthesis | −2.2 ± 0.5 | −1.6 ± 0.4 | 1.4 |
| NM_011300 | Ribosomal protein S7 | Rps7 | Protein biosynthesis | −2.0 ± 0.4 | −1.5 ± 0.6 | 1.4 |
| NM_026405 | RAB32, member RAS oncogene family | Rab32 | Protein transport | −2.4 ± 0.4 | −1.9 ± 0.4 | 1.3 |
| NM_017478 | Coatomer protein complex, subunit gamma 2 | Copg2 | Protein transport | −2.3 ± 0.6 | −2.0 ± 0.9 | 1.2 |
| NM_030559 | Vacuolar protein sorting 16 (yeast) | Vps16 | Intracellular protein transport | −2.1 ± 0.7 | −1.7 ± 0.6 | 1.2 |
| NM_029782 | Calreticulin 3 | Calr3 | Protein folding | −2.2 ± 0.5 | −1.9 ± 0.6 | 1.2 |
| NM_025592 | Ribosomal protein L35 | Rpl35 | Protein biosynthesis | −2.0 ± 0.6 | −1.8 ± 0.6 | 1.1 |
| NM_024212 | Ribosomal protein L4 | Rpl4 | Protein biosynthesis | −2.2 ± 0.5 | −2.0 ± 0.6 | 1.1 |
Definition of abbreviations: GO, Gene Ontology Consortium; Mt, metallothionein.
Values are mean fold changes compared to corresponding nonexposed control values ± SEM, with an Mt1/2(+/+) to Mt1/2(+/+) expression ratio. Genes were considered statistically significant if average intensity > 300 and false discovery rate ⩽ 0.1. Gene ontology biological processes were derived from the Gene Ontology Consortium (MGI).