TABLE 3.
EXPRESSION DIFFERENCES IN GENES INVOLVED IN THE INFLAMMATORY RESPONSE, CHEMOTAXIS, EXTRACELLULAR MATRIX REGULATION, AND COAGULATION AND FIBRINOLYSIS IN THE LUNGS OF GENE-TARGETED MICE LACKING METALLOTHIONEIN 1 AND 2 [Mt1/2(−/−)] AND 129S1/SvImJ STRAIN-MATCHED WILD-TYPE MICE [Mt1/2(+/+)]EXPOSED TO NICKEL FOR 72 h
| Accession ID | Gene Name | Symbol | Mt1/2(+/+) Fold Change | Mt1/2(+/+) Fold Change | Mt1/2(−/−)/Mt1/2(+/+) Ratio | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Inflammatory Response/Chemotaxis | ||||||||||
| NM_011330 | Small chemokine (C-C motif) ligand 11 | Ccl11 | 6.9 ± 1.7 | 2.4 ± 0.6 | 2.9 | |||||
| NM_009704 | Amphiregulin | Areg | 7.9 ± 2.4 | 3.6 ± 1.0 | 2.2 | |||||
| NM_011332 | Chemokine (C-C motif) ligand 17 | Ccl17 | 8.3 ± 1.8 | 3.9 ± 0.7 | 2.1 | |||||
| NM_007577 | Complement component 5, receptor 1 | C5r1 | 3.4 ± 0.7 | 1.7 ± 0.4 | 2.0 | |||||
| NM_009140 | Chemokine (C-X-C motif) ligand 2 | Cxcl2 | 5.2 ± 2.0 | 2.7 ± 1.0 | 1.9 | |||||
| AI323594 | Chemokine (C-C motif) ligand 2 | Ccl2 | 23.5 ± 12.4 | 12.8 ± 6.3 | 1.8 | |||||
| NM_020509 | Resistin-like α | Retnla | 11.9 ± 3.0 | 7.9 ± 1.8 | 1.7 | |||||
| NM_008361 | IL-1β | Il1b | 6.8 ± 2.0 | 4.5 ± 1.4 | 1.5 | |||||
| NM_015783 | INF-α–inducible protein | G1p2 | 2.7 ± 1.0 | 2.0 ± 0.7 | 1.4 | |||||
| NM_010798 | Macrophage migration inhibitory factor | Mif | 2.5 ± 0.8 | 1.8 ± 0.5 | 1.4 | |||||
| NM_013611 | Nodal | Nodal | 2.2 ± 0.5 | 1.6 ± 0.4 | 1.4 | |||||
| NM_019568 | Chemokine (C-X-C motif) ligand 14 | Cxcl14 | 3.3 ± 0.6 | 2.5 ± 0.4 | 1.3 | |||||
| NM_009402 | Peptidoglycan recognition protein 1 | Pglyrp1 | 3.0 ± 0.6 | 2.3 ± 0.5 | 1.3 | |||||
| NM_008336 | INF-α family, gene B | Ifnab | 2.2 ± 0.4 | 2.0 ± 0.4 | 1.2 | |||||
| NM_008334 | INF-α family, gene 7 | Ifna7 | 2.1 ± 0.5 | 1.7 ± 0.4 | 1.2 | |||||
| M58004 | Chemokine (C-C motif) ligand 6 | Ccl6 | 2.0 ± 0.5 | 1.7 ± 0.4 | 1.2 | |||||
| Extracellular Matrix | ||||||||||
| NM_010217 | Connective tissue growth factor | Ctgf | 4.3 ± 1.2 | 1.7 ± 0.5 | 2.5 | |||||
| X56304 | Tenascin C | Tnc | 31.2 ± 11.0 | 15.1 ± 5.3 | 2.1 | |||||
| NM_013599 | Matrix metalloproteinase 9 | Mmp9 | 3.1 ± 0.6 | 1.6 ± 0.3 | 1.9 | |||||
| NM_011593 | Tissue inhibitor of metalloproteinase 1 | Timp1 | 29.2 ± 6.0 | 16.3 ± 3.3 | 1.8 | |||||
| NM_009928 | Procollagen, type XV | Col15a1 | 2.2 ± 0.3 | 1.5 ± 0.2 | 1.5 | |||||
| NM_011580 | Thrombospondin 1 | Thbs1 | 26.7 ± 8.6 | 19.7 ± 6.4 | 1.4 | |||||
| D28599 | Chondroitin sulfate proteoglycan 2 | Cspg2 | 9.9 ± 2.5 | 7.5 ± 1.9 | 1.3 | |||||
| NM_009369 | Transforming growth factor β–induced | Tgfbi | 2.9 ± 0.5 | 2.2 ± 0.4 | 1.3 | |||||
| NM_008608 | Matrix metalloproteinase 14 (membrane-inserted) | Mmp14 | 4.1 ± 0.7 | 3.3 ± 0.6 | 1.2 | |||||
| NM_008485 | Laminin γ2 | Lamc2 | 2.9 ± 0.5 | 2.4 ± 0.4 | 1.2 | |||||
| NM_009614 | A disintegrin and metalloproteinase domain 15 (metargidin) | Adam15 | 2.0 ± 0.4 | 1.6 ± 0.3 | 1.2 | |||||
| NM_007925 | Elastin | Eln | 4.0 ± 0.9 | 3.6 ± 0.9 | 1.1 | |||||
| NM_007993 | Fibrillin 1 | Fbn1 | 2.1 ± 0.4 | 2.0 ± 0.4 | 1.1 | |||||
| Coagulation and Fibrinolysis | ||||||||||
| NM_008871 | Serine (or cysteine) proteinase inhibitor, clade E, member 1 | Serpine1 | 14.1 ± 7.6 | 4.4 ± 1.8 | 3.2 | |||||
| NM_008872 | Plasminogen activator, tissue | Plat | 8.7 ± 2.0 | 4.9 ± 1.2 | 1.8 | |||||
| NM_011113 | Urokinase plasminogen activator receptor | Plaur | 3.8 ± 0.8 | 2.8 ± 0.6 | 1.4 | |||||
Definition of abbreviation: Mt, metallothionein.
Values are mean fold changes over corresponding nonexposed control values ± SEM, with a Mt1/2(+/+) to Mt1/2(+/+) expression ratio. Genes were considered statistically significant if average intensity > 300 and false discovery rate (FDR) ⩽ 0.1.