Figure 1.

Chronic EtOH ingestion increased angiotensinogen, AT₁, and AT₂ expression as well as ACE activity in the lung. Rats were fed Control or EtOH diets for 6 wk. In A and B, equivalent amounts of lung homogenates were subjected to SDS-PAGE; probed with primary antibodies to the RAS components angiotensinogen, renin, angiotensin converting enzyme (ACE), AT₁, and AT₂; and quantified by laser densitometry. In B, each bar represents the mean relative density in arbitrary units ± SEM as % Control. Open bars, control; filled bars, EtOH; n = 5; *P < 0.05 versus Control. Representative Western blots are shown in A. In C, equivalent amounts of lung homogenates were subjected to ACE activity assay. Each bar represents the mean ± SEM (n = 3–4, *P < 0.05 versus Control).