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. 2005 Dec 9;34(3):330–337. doi: 10.1165/rcmb.2005-0101OC

Figure 3.

Figure 3.

DMT1 expression in human tissues. Human MTE blot probed for DMT1 mRNA. A human MTE blot (Clontech) was probed with a randomly primed 32P-labeled 1.1-kb fragment of human DMT1 (non–isoform-specific). Loading of mRNA was normalized by the manufacturer to several housekeeping genes (β-actin, glyceraldehyde 3′- phosphate dehydrogenase, ubiquitin, 23-kD highly basic protein, β-tubulin, phospholipase A2, ribosomal protein S9, transferrin receptor, and hypoxanthine guanine phosphorbiosyl transferase). Radioactivity was measured by phosphorimaging; shown are phosphor counts quantified using Quantity One software (BioRad).