Table 2.
Hybridizes to bit set to 0 | Hybridizes to bit set to 1 | ||
---|---|---|---|
a0 | AGAATTGAGTAAGAG | a1 | TAAGTAATGTGAGGA |
b0 | gttatTAAGAGGTTGATATG | b1 | gttatGGATATAAAGGAAGT |
c0 | TGTGAAGTGGAGGAT | c1 | GGATGTTTGTTATAA |
d0 | gtaaaGAGGGAAGATTTTAA | d1 | gtaaaTGAAGAGGGTTATGT |
e0 | GGTGTGGATAAATAG | e1 | TATGGAAAGTAAGGT |
f0 | GGAATTGTTTGAAGC | f1 | GTAGGGAGAATGTAC |
g0 | attgaTTGAATTTGAGAGTT | g1 | attgaGAATATAAGATTATG |
h0 | TGAAGTAAAGGTTAG | h1 | GAAGTATGTGATTAT |
i0 | GTGGGATAAGGAATG | i1 | TAGGTAGTTGGTGGA |
Twenty-base oligonucleotides are complementary to both the 15-nt bit sequence (uppercase) and adjacent 5-nt spacer (lowercase) to increase the melting temperatures of weaker hybridizing oligonucleotides.