Figure 2.

Effect of thapsigargin on ΔF508 CFTR expression and interaction with calnexin. (A) ΔF508 CFTR–expressing BHK cells were treated with 10 μM thapsigargin for 0, 1.5, or 24 h (+) or left untreated (−). Western blot of lysates, including untreated WT, is shown. (B) Cells were grown in 1 or 10 μM thapsigargin for 1.5 or 24 h. ΔF508 CFTR was immunoprecipitated from the cell lysates and immunoblotted with anti-CFTR as in A. (C) Same blot probed with anti-calnexin, showing that amount of CFTR-associated calnexin reflects amount of CFTR.