Figure 3.

Neutrophil chemokinesis is not altered by CF ASL. (A) Low-power view of neutrophils injected into a droplet of ASL. (B) Isolated neutrophils have settled down after being injected into the ASL droplet and are captured at the start (T = 0) of the experiment. (C) Positions of the same neutrophils after 25 min (T = 25 min). Movements of the five labeled neutrophils are illustrated with dotted white lines. (D) Quantitative velocities of neutrophils when stimulated (+) or not stimulated (−) with fMLP in PBS, normal (WT) ASL or cystic fibrosis (CF) ASL from MilliCell culture wells. (E) Quantitative velocities of unstimulated (−) or fMLP-stimulated (+) neutrophils in PBS, normal (WT) ASL, or CF ASL from Transwell culture wells. Data shown as mean ± SEM are from four independent experiments conducted on different days with cells from different donors.