Figure 6.

Respiratory burst in neutrophils is not altered by CF ASL. (A) Fluorescence measurement of reactive oxygen species (ROS) production by neutrophils, when PMA-stimulated (black curve), or quiescent (gray curve filled underneath). The light-scattering properties of the neutrophil population are illustrated by the inserted dot plot. (B) Graphical quantification of DHR fluorescence of PMA-stimulated (+) or quiescent (−) neutrophils, in PBS, normal (WT) ASL, or CF ASL. Data are shown as mean ± SEM and are from five independent experiments.