Figure 5.

Proteosomal inhibition moderately increases 5-LO expression; the effect is less than that of 1 μM GSNO, and is additive with that of 1 μM GSNO. (A) Western blot analysis was performed on whole cell extracts from A549 cells treated with the protesome inhibitor, clasto-lactacystin β-lactone (25 μM), in the presence or absence of 1 μM GSNO. The membrane was stripped and reprobed with α-tubulin to verify that an equal amount of protein was loaded. Twenty-five micrograms of protein was loaded in each lane (n = 3; P < 0.003). (B) Blots were scanned and densitometry was performed for quantification.