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Pifithrin blocks asbestos-induced ΔΨm and apoptosis. A549 cells were treated with pifithrin (30 μM × 24 h) and exposed to doxorubicin (500 ng/ml) or asbestos (25 μg/cm2) for 24 h. ΔΨm (A) and TUNEL staining (B) were determined as described in Materials and Methods. Data are expressed as mean ± SEM. *P < 0.05 versus control; †P < 0.05 versus (−) pifithrin (n = 6).
