TABLE 1.

PIFITHRIN BLOCKS ASBESTOS-INDUCED AT2 CELL APOTOSIS AND CASPASE 9 ACTIVATION^*

Condition	TUNEL (% positive cells at 24 h)	DNA Fragmentation (fold control at 24 h)	Caspase 9 (% control RU at 24 h)
Control	2.6 ± 0.6	1.0 ± 0	100 ± 0
Pifithrin (30 μM)	4.1 ± 0.8	0.9 ± 0.3	110 ± 10
Doxorubicin (500 ng/ml)	60.6 ± 4.6†	5.2 ± 1.7†	240 ± 30†
Asbestos (25 μg/cm2)	39.9 ± 2.0†	4.3 ± 1.0†	200 ± 30†
Pifithrin + doxorubicin	25.7 ± 2.0†‡	1.9 ± 0.2†‡	140 ± 10†‡
Pifithrin + asbestos	18.0 ± 1.9‡	1.3 ± 0.3‡	130 ± 20‡

^*n = 6 for each group.

^†P < 0.05 versus control.

^‡P < 0.05 versus doxorubicin or asbestos.