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. Author manuscript; available in PMC: 2010 Feb 20.
Published in final edited form as: J Mol Biol. 2009 Jan 3;386(2):300–315. doi: 10.1016/j.jmb.2008.12.064

Table 5.

L4 mutant growth rates and macrolide sensitivitya

L4 allele Doubling time (min) ERY doubling time (min) ERY CDIC (μg/mL) SPIR doubling time (min) SPIR CDIC (μg/mL)
wild-type 31 151 200 149 750
Gln62Phe 47 63 1000 79 2500
Gln62Leu 44 63 1125 78 2500
Gln62Met 41 56 1125 60 1875
Gln62Asp 43 58 1000 75 2500
Gln62Glu 39 55 1000 78 1875
Gln62Lys 42 64 1125 66 1875
Gln62Arg 43 63 1125 75 2500
Lys63Glu 44 61 1250 68 1750
Lys63Asp 44 69 1000 104 1000
Lys63Leu 36 65 750 77 2000
Lys63Ala 34 58 500 90 1000
Lys63Val 34 59 500 92 875
Lys63His 37 59 1000 53 2000
Lys63Ile 46 68 1250 NDb NDb
Lys63Gln 37 59 750 NDb NDb
Gly66Cys 38 57 1250 56 2500
Gly66Ser 33 55 1000 55 1750
Gly66Ala 34 55 875 74 1250
Gly66Asp 32 56 875 69 1500
Gly66His 39 56 1000 54 1750
Gly66Arg 42 61 1250 56 2250
a

Strains were grown in LB media at 37 °C with aeration. Growth rates were determined in the presence of erythromycin (ERY) at 200 μg/mL and spiramycin (SPIR) at 750 μg/mL, and expressed as the time required for cell mass to double during logarithmic growth. Macrolide cell-doubling inhibitory concentrations (CDIC) were determined as described in Materials and Methods.

b

Not determined.