Table 6.
Plasmid-borne L4 mutant growth rates and erythromycin sensitivitya
| L4 allele | Doubling time (min) | ERY doubling time (min) | ERY CDIC (μg/mL) |
|---|---|---|---|
| pS10(wild-type) | 33 | 127 | 200 |
| pS10(Thr65Pro) | 32 | 95 | 250 |
| pS10(Gly64Cys) | 34 | 115 | 200 |
| pS10(Gly64Val) | 36 | 83 | 750 |
| pS10(Gly64Ser) | 34 | 128 | 200 |
| pS10(Gly64Cys-Thr65Pro) | 35 | 43 | 1500 |
| pS10(Gly64Val-Thr65Pro) | 39 | 62 | 1000 |
| pS10(Gly64Ala-Thr65Pro) | 33 | 43 | 2000 |
| pS10(Gly64Ser-Thr65Pro) | 34 | 45 | 2000 |
| pS10(Gly64Arg-Thr65Pro) | 39 | 56 | 1500 |
| pS10(Gly64Asp-Thr65Pro) | 37 | 48 | 2000 |
a
Strains were grown in LB media at 37 °C with aeration. Growth rates were determined in the absence and presence of erythromycin (200 μg/mL), and expressed as the time required for cell mass to double during logarithmic growth. Erythromycin cell-doubling inhibitory concentrations (CDIC) were determined as described in Materials and Methods.