Figure 4.
Expression of candidate genes, including CCR5, observed by microarray analysis was validated by qRT-PCR. Quantitative RT-PCR analysis was performed (in duplicate) for six significantly regulated genes: CCR5, fibronectin, GRP78, peroxiredoxin, STAT2, and a gene encoding protein of unknown function, and the data compared as log2(treated/control) to their corresponding microarray expression profiles. The temporal expression profiles for all strains show a positive correlation to values measured by qRT-PCR.