TABLE 1.
SUMMARY OF GENES SIGNIFICANTLY EXPRESSED DUE TO OVALBUMIN TREATMENT
| Phenotype
|
Significant Genes
|
|||
|---|---|---|---|---|
| Strain | AHR | EOS | Up | Down |
| 129/SvIm | + | + | 209 | 274 |
| C57BL/6J | − | + | 132 | 117 |
| BALB/cJ | + | − | 427 | 135 |
| CAST/Ei | − | − | 40 | 217 |
Definition of abbreviations: AHR, airway hyperresponsiveness; EOS, eosinophil.
Genes with statistically significant changes were determined using SAM, an algorithm whereby temporal changes in gene expression for each strain were calculated using a two-class paired analysis. Normalized Log2ratios (n = 3–4) from samples precontrol and post–ovalbumin (OVA) challenge for individual time points of 24, 48, 72 h were obtained. Results include gene expression changes due to OVA challenge in treated (OVA) and control (alum) animals, and lists reflect unique genes only corrected for gene duplicate found in our microarray clone sets.