TABLE 2.
mRNA CONTENT FOR GENES OF CLUSTER 1
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Microarray Relative Abundance Induction
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Microarray Fold Induction
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RT-PCR Fold Induction
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|---|---|---|---|---|---|---|
| Functional Category | Gene Symbol | Common Name | (Mean f.u.) | (Mean ± SE) | (Mean ± SE) | Hormonal Response |
| Surfactant-related | SFTPC | SP-C | 5137 | 123.8 ± 25.2 | 42.5 ± 18.9 | Synergy* |
| SFTPA | SP-A | 4507 | 145.6 ± 58.0 | 18.9 ± 7.2 | DEX-permissive* | |
| PGC | Pepsinogen C | 1385 | 49.7 ± 15.2 | 192.4 ± 4.1 | Synergy* | |
| SFTPB | SP-B | 8190 | 17.1 ± 4.2 | 37.2 ± 15.5 | Synergy | |
| LAMP3 | Lysosomal protein | 1396 | 13.8 ± 4.3 | 13.4 ± 6.2 | Synergy | |
| LPL | Lipoprotein lipase | 337 | 10.7 ± 1.0 | 23.3 ± 9.1 | Synergy* | |
| Regulatory | WIF1 | Wnt inhibitory factor 1 | 421 | 12.9 ± 3.6 | 16.3 ± 4.5 | cAMP only |
| IGF2 | Insulin-like growth factor 2 | 879 | 10.7 ± 3.1 | 26.3 ± 20.1 | DEX only | |
| IL1RL1 | IL-1 receptor-like 1 | 105 | 7.2 ± 0.7 | 19.0 ± 3.6 | Synergy | |
| NR4A2 | Nuclear receptor 4A2 | 147 | 6.1 ± 1.2 | 4.8 ± 0.6 | cAMP only | |
| HIF3A | Hypoxia induced factor 3α | 57 | 7.7 ± 1.4 | 30.7 ± 36.1 | cAMP only | |
| Metabolic | MAOA | Monoamine oxidase A | 454 | 8.6 ± 4.0 | 11.8 ± 3.5 | cAMP-permissive* |
| ADH1B | Alcohol dehydrogenase 1β | 165 | 8.2 ± 1.5 | 26.3 ± 10.1 | DEX only* | |
| SEPP1 | Selenoprotein P | 267 | 3.2 ± 0.4 | 6.0 ± 0.9 | Synergy | |
| Transport | SCNN1A | Epithelial sodium channel-α | 966 | 17.0 ± 2.5 | 23.6 ± 8.7 | Synergy* |
| CLDN18 | Claudin 18 | 750 | 9.8 ± 3.5 | 15.5 ± 8.2 | DEX-permissive | |
| AQP4 | Aquaporin 4 | 321 | 6.9 ± 0.8 | 5.9 ± 3.8 | Additive* |
Definition of abbreviations: cAMP, cyclic AMP; DEX, dexamethasone; f.u., fluorescence units; SP, surfactant protein; Wnt, Wingless-Int.
Data from Ref. 4.
Genes of Cluster 1 as identified by self-organizing map analysis are shown by functional categories. Fold induction data are mean ± SE from 5 (microarray) and 3 (RT-PCR) experiments with exposure of cells to dexamethasone/8-Br-cAMP/isobutylmethylxanthine (DCI) for 72 h. Hormonal response data were obtained using RNA from cells exposed to DEX alone, cAMP/isobutylmethylxanthine (cAMP) alone, DCI, or untreated with analysis by RT-PCR or microarray (7). A DEX-permissive effect is defined as no response to DEX alone but increased responsiveness to cAMP in the presence of DEX compared to cAMP alone. A cAMP-permissive effect reflects the opposite response to the two hormones.