Table 1.

Primer pair and amplicon information for genes of interest and housekeeping gene (GAPDH)

	GenBank No.	Amplicon Size, bp	Annealing Temperature, °C	mRNA Region, bp
Collagen type I	NM_000088	112	60	207–318
5′-GGCCAAGACGAAGACATCCCACCAA-3′ (sense)
5′-TGCCGTTGTCGCAGACGCAGAT-3′ (antisense)
Collagen type III	NM_000090	124	60	1400–1523
5′-GAGGTGGTGCAGGTGAGCCTGGTAA-3′ (sense)
5′-GATCCATCCTTGCCATCTTCGCCTT-3′ (antisense)
Decorin	NM_009120	116	60	954–1069
5′-CACCAATCCGCTGAAGAGCTCAGGAA-3′ (sense)
5′-GAGGAAGACCTTGAGGAATGCTGGTGATA-3′ (antisense)
Biglycan	NM_001711	103	60	741–843
5′-GAACTGCATCGAGATGGGCGGGAA-3′ (sense)
5′-CTTGGCCTCTGAGATGCGCAGGTA-3′ (antisense)
Fibromodulin	NM_002023	108	60	891–998
5′-GCTAGACCTCTCCTACAACCAGCTGCAGAA-3′ (sense)
5′-AAGCTGCTGATGGAGAACTCATTGATCCTA-3′ (antisense)
Versican (V1)	NM_004385	112	60	1209–1320
5′-TGTGGAGGTGGTCTACTTGGGGTGAGAA-3′ (sense)
5′-CACTCATTCGACGTTTAAAGCAGTAGGC-3′ (antisense)
MMP-2	NM_004530	103	60	2097–2199
5′-TGGATCCTGGCTTCCCCAAGCTCAT-3′ (sense)
5′-AAGAAGTAGCTGTGACCGCCGCCCT-3′ (antisense)
MMP-9	NM_004994	94	60	1176–1269
5′-GCTTCTGCCCGGACCAAGGATACAGTT-3′ (sense)
5′-GCCTCCGGCACTGAGGAATGATCTAA-3′ (antisense)
MMP-3	NM_002422	89	59	671–759
5′-ATGGACAAAGGATACAACAGGGACCAA-3′ (sense)
5′-TGTTGGCTGAGTGAAAGAGACCCAGG-3′ (antisense)
TIMP-1	NM_003254	131	60	410–540
5′-AAGCCTTAGGGGATGCCGCTGACAT-3′ (sense)
5′-GAGTCCATCCTGCAGTTTTCCAGCAAT-3′ (antisense)
GAPDH	NM_002046	114	59	10–123
5′-CCTCCTGTTCGACAGTCAGCCGCAT-3′ (sense)
5′-GCGCCCAATACGACCAAATCCGTT-3′ (sense)

MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase.