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. Author manuscript; available in PMC: 2009 Nov 7.
Published in final edited form as: Mol Cell. 2008 Nov 7;32(3):394–405. doi: 10.1016/j.molcel.2008.09.017

Figure 6. The KEN Boxes of Bub1 Are Required for the Spindle Checkpoint.

Figure 6

(A) HeLa tet-on cells stably expressing RNAi-resistant mCherry-Bub1 wild-type (WT) or ΔKEN were transfected with Bub1 siRNA and then treated with Taxol. The cell lysates were blotted with the indicated antibodies. The positions of mCherry-Bub1 and the endogenous (Endo.) Bub1 are indicated.

(B) Cells described in (A) were incubated with Hoechst 33342 for 30 min and visualized using an inverted fluorescence microscope. The DNA morphology is shown in the left panels. The overlay of DNA in cyan and differential interference contrast (DIC) in red is shown in the right panels.

(C) The mitotic indices of the cells described in (A) and (B). About 1000 cells from three independent experiments were counted. The averages and standard deviations are shown.