Figure 3.
Expression of TH in the ventral tegmental area after systemic injections of saline or cocaine with Coptidis rhizoma and Berberine. The results are presented as the mean ± SE. The total number of TH-like immunoreactive neurons within a (50 × 50 μm) grid over the areas at × 400 magnification. Significance with Tukey's test following a one-way ANOVA is indicated as ***P < 0.001 versus SAL group and CON group, or ###P < 0.001 and +++P < 0.001 versus COC group. The cells within the tegmental cell area were counted on each of three sections per animal.