Figure 3.

Nrf1 interacts with MCRS2 in vivo. Top row, the transfected expression plasmids are indicated. Total cell extracts were prepared from the cells transfected with the indicated expression plasmids and were then incubated with an antibody against FLAG epitope (lane 1–6) or a pre-immune serum (lane 7). The resulting co-immunoprecipitates were separated on a 10% SDS-PAGE gel and probed with an antibody against HA epitope. The control experiments (middle and bottom rows) were done using the same cell extracts as the top row to indicate the transfected cell indeed express FLAG fusion protein (middle row) and HA fusion protein (bottom row) and were detected by anti-FLAG or anti-HA antibody, respectively. IgG HC, immunoglobulin heavy chain. WB, western blot. PS, pre-immune serum.