Table 1.
Listing of markers used to establish the identity of cortical structures in immature oocytes.
| Name | Type | Target | Reference | Present in a cortical structure |
| CDC2 | mouse monoclonal antibody | Cell division cycle 2, catalytic subunit of MPF | [22] | + |
| PSTAIR | mouse monoclonal antibody | Cyclin binding domain in all Cdks | [23] | + |
| SEC23 | goat polyclonal antibody | SEC23, component of the coat protein II complex | [18] | + |
| P-GM130 | rabbit polyclonal antibody | GM130 phosphorylated on serine 25 | [27] | + |
| Calnexin | rabbit polyclonal antibody | Calnexin, transmembrane protein | [26] | - |
| Gamma-tubulin | rabbit polyclonal antibody | Gamma-tubulin, cytoskeletal component | [25] | - |
| Mitotracker* | Fixable live cell dye | Oxidation within mitochondria | [28] | - |
| NUP153 | mouse monoclonal antibody | Nucleoporin 153 kDa | [29] | - |
| GM130 | mouse monoclonal antibody | Structural Golgi protein | [27] | - |
| Cyclin B | mouse monoclonal antibody | Cyclin B, regulatory subunit of MPF | [22] | - |
| SPDY | rabbit polyclonal antibody | SPDY, regulatory protein of CDC2 | [19] | + |
* Stained by 30 min incubation in maturation medium prior to fixation.