Table 3.

Alterations (P values) in gene expression level of PGK1 by different reference genes' normalizations

Reference gene	Totala	Normal/TMa	Normal/TNMa	TM/TNMb
TBP and HPRT1	0.005	0.002	0.338	0.027
TBP	0.030	0.017	0.868	0.024
HPRT1	0.002	0.001	0.263	0.016
RPL13A	0.004	0.004	0.748	0.008
GAPDH	0.054	0.024	0.806	0.043
18S	0.343	0.717	0.312	0.504
ACTB	0.385	0.792	0.995	0.782

a: One-Way ANOVA

b: Paired-samples t test