Figure 4. The specificity of Δ410 suppression to Nef expression over Tat and Rev.
A & C. The secondary structure of 5′UTR and 3′UTR of all minigenes were predicted by the RNAfold program. The length in nucleotides for each 5′UTR and 3′UTR was given beneath each minigene and mutants. B & D. 293T cells were transfected with each of the minigenes, Sam68 or each of its mutants. Cells were harvested to determine Tat, Rev, or Nef expression by Western blot analysis.