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. Author manuscript; available in PMC: 2010 Jan 16.
Published in final edited form as: Mol Cell. 2009 Jan 16;33(1):87–96. doi: 10.1016/j.molcel.2008.11.024

Table 1.

Δ410 binding to 3′UTR of nef mRNA

Hybrid protein 1 Hybrid RNA Hybrid protein 2 Relative β-Gal activity
MS2-coat MS2 IRP 0
MS2-IRE IRP 6.763 ± 0.429
MS2 Δ410 ND
MS2-nef 3′UTR Δ410 0.122 ± 0.025
MS2-nef 3′RTU Δ410 ND
MS2 Δ269 ND
MS2-nef 3′UTR Δ269 ND
MS2-nef 3′RTU Δ269 ND

The yeast strain L40 was transfected with hybrid protein 2 and hybrid RNA expression plasmids as indicated. The transformants were grown on an SD plate lacking tryptophan, leucine, uracil, and histidine for 5 days. Fifteen colonies from each transformation were inoculated into the same SD liquid medium, triplicates of each inoculation were determined for the β-Gal gene expression using the liquid culture β-Gal assay. Data are mean ± SEM. ND: no detectable β-Gal activity or no colony growth.